LOCUS       40924_21613        6221 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pGFpsd-hph DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Sato M, Kurahashi A, Nishibori K, Fujimori F.
  TITLE     Development of a transformation system for the edible mushroom 
            Grifola frondosa: demonstrating heterologous gene expression and 
            RNAi-mediated gene silencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Sato M, Kurahashi A, Nishibori K, Fujimori F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2014) Contact:Masayuki Sato Yukiguni Maitake Co. 
            Ltd., Research and Development; 89 Yokawa, Minamiuonuma, Niigata 
            949-6695, Japan URL :http://www.maitake.co.jp
REFERENCE   3  (bases 1 to 6221)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6221)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-FEB-2014) Contact:Masayuki Sato Yukiguni Maitake Co. Ltd., 
            Research and Development; 89 Yokawa, Minamiuonuma, Niigata 949-6695,
            Japan URL :http://www.maitake.co.jp"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..1500
                     /gene="Gf.PSD1"
                     /regulatory_class="promoter"
     gene            1..1500
                     /gene="Gf.PSD1"
                     /label=Gf.PSD1
     CDS             1501..2523
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      2527..3531
                     /gene="Gf.PSD1"
                     /regulatory_class="terminator"
     gene            2527..3531
                     /gene="Gf.PSD1"
                     /label=Gf.PSD1
     primer_bind     complement(3579..3595)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3603..3619)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3627..3657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3672..3693)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3981..4569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4743..5600)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5601..5705)
                     /label=AmpR promoter
     primer_bind     6179..6195
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgtccaacgt tggctctgct ccatcatatg cgtggggatg cgtccaatat atccaatttc
       61 tcagtaccaa cccctctcgg gcgcagaaag agtcgtcacg tcaatgatac ttcaacgatt
      121 tgagagcttt taatattccg tgagtgttgt taattcactg catcaatgat ggacacttgg
      181 attttaatga tcgtcctggg ttaaggcttc ggggacctgc cagcacccac aagactatga
      241 aagccgcctc ctattggtct agctcccgaa ccatatgaga tactaacgtt tcgcattttt
      301 accacatcgc tgcaggctcg tatacgcatt atttgctcat tctccagtgc atgaagtttt
      361 ggttatgtcg agcacgatga cttcaatggc cttgtatcgc cgatcaaaca cctgggcagt
      421 cgacgaagtc ccggtctcag gacgaagttc caaccgtgca ggacggttat caaactccat
      481 cgatctgctt ctgatgtgtc tgtattggcc taatctgagc gacaatgttg tcattcagcc
      541 acatgaagct gggcgctccc gaacagtgga cagagagaag gattggcatg ttctgcttcc
      601 cacttcgcgc cgcctccaga cgtcaacata gttcgacgta accttggatg aggaacttga
      661 ctgaatgcat ccaacgggct ttcaccttct ctttaccaaa cagtgcattc tgtcacaata
      721 ggttcgccgc acttttatgc agcgctgatg tccgttgcag tggccggtct tgaatacaat
      781 ttcctgactt tctacctctt ggccgtcgtc cgtggcgctc gcactttctt tccacaggtg
      841 taatgatcat tcctcagggc ttgattacaa tgaccgggca cgatgcaggg aaaaacgttt
      901 gaatgtaccg gacatgctct tcacgcttct gtttgctgac ctggtcagaa ggcttcagaa
      961 ggtcccgtct acaaagcaat tacagcatat tccatgcaat gaatggtcac gtatgcccct
     1021 tcctgtctac ttattcggca cactcaacga tatgagccac cctccgtgga actatgcatt
     1081 gctctttacg atgctgtctt gcaacgttcc tcagttgttg tgttcgcttc gtcttccatg
     1141 atcaaccaat caccgttctg gcctctgacg attcacaggg tacgcgattc tggacaagtt
     1201 agcaccattc ggtatcagac ggagttgcac agttgtagaa tcatccaatg ttcttcacct
     1261 aggccgtctt aatcacgttg caaatagcgg ctctggctgg gtcctcatga cgttactgcg
     1321 accgcacata ttccctccgt gatacctttc ctctcgcata aaaagatccc atcccatctc
     1381 tccgtcccat atcgaaactt tcctctccct ggtgactcac aaacgcagtg actcttgaaa
     1441 gtctgccctc gttgcttctt tctcgtatgc gctcacgctg acatttctcc ctccagagca
     1501 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     1561 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     1621 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     1681 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     1741 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     1801 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     1861 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     1921 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     1981 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     2041 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     2101 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     2161 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     2221 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     2281 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     2341 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     2401 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     2461 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     2521 gaatagggtg ccgttatcgc tcacaaggtc tgggcttgaa agttgtctcg ttcacatatc
     2581 tgtaggaata tctgacggag aaacgtatgc acccatgtat tattaccagt tgacgttagc
     2641 catgtacgca gtgcaaagtt gttgtgggac aacttctttg tggtgatcgg ggctaacgag
     2701 ccatggccgg cgcggtgtcg cttgtcctcc catctggtta gattatcgga gagttcctgc
     2761 gcacggttga cgatagtgag ttgtccggat aaaaaatctg gccttccgcg cgccgaaggc
     2821 cgtattgtcc ggattaatca ggcatgaggg gtccaaaatc ccaaagtttg tccgaatttc
     2881 catcacccct gctctcccat tatcaggggc cctttgacga tctgcttgac ggattcgctg
     2941 cgccttttgt cgagcacgtg tctcgacgtt cttcatcctc tctgtctgcg tcccttcgaa
     3001 ggcgaagtcg taaagcacgt cgcccgcagg ttcagaattt tgagcggtca cgttcgggga
     3061 tgacggccga tacacgcgtc gcaacaacca cctccggaga tgccatacaa ttgactgttc
     3121 tcggaaattc catgctgtta tggccatgtc ccaaatcggc cagtccgcca gggagagtac
     3181 gatggcggaa gaacacggac gcgaaaccac ttccacaaag cagctcgtaa tacggaaggt
     3241 gtcgggacgc agcctttaga gttaactgcg gtctcgtttc ttcatcgtgg tttccacacg
     3301 cggttggcag atagaagtcg ggcgtgctgt gcaatccaaa tatatcagcg attcgctctg
     3361 accgcagcgc cgaacaaatg atctcgagtc ctctgacctt ccaaactgcg gataacagtt
     3421 tttacgtccg cgcttttagt gctgagcatt tcgaataagg cattcatatt gatatgaacc
     3481 ttgccgatcg ataccaatcc tgggatggtt agacggcaga tgggggaaca tgatcctcta
     3541 gagtcgacct gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     3601 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     3661 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     3721 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     3781 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3841 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3901 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3961 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4021 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4081 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4141 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4201 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4261 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4321 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4381 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     4441 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4501 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4561 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4621 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4681 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4741 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4801 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4861 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4921 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     4981 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     5041 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     5101 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     5161 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     5221 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     5281 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     5341 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     5401 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     5461 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     5521 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     5581 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     5641 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5701 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     5761 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     5821 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     5881 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     5941 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     6001 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     6061 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     6121 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     6181 aaaacgacgg ccagtgaatt cgagctcggt acccggggat c
//
